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Exercise as a modifier of glycidic and lipid metabolism in sleep-deprived mice


Repeated deprivation of a fundamental need, such as sleep, results in physiological adaptations and phenotypic changes. Sleep deprivation (SD) is known to be associated with adverse consequences for individual health and well-being. Weight management and glycemic control are negatively affected by SD. To date, in vivo systemic and local metabolic shift patterns associated with SD and the modifying factors need more clarification.


To evaluate metabolic alterations associated with SD in mice and to further investigate the influence of associated exercise


Three-month-old swiss mice were divided into four groups: control, SD, exercise (EX) and previous exercise followed by SD (EX+SD). Exercised animals were submitted to motorized treadmill (9m / s) for 8 weeks. Sleep deprived mice were submitted to the modified multiple platform model (72h). The metabolite profiles of mice cortex, in all groups, were analyzed using liquid chromatography/mass spectrometry. Data were expressed as Mean ± Standard Error of Mean (E.P.M.). ANOVA, followed by Newman-Keuls test was performed and significance was considered at p <0.05 . Statistical analysis was performed using GraphPad Prism® 5.01 software.


EX mice showed high levels of serum glucose (144.40 ± 8.27 mg/dL), lactate (98.50 ± 4.56 mg/dL) and triglycerides (227.10 ± 28.03 mg/dL) (p<0.05). Serum triglycerides were reduced in mice with SD (72.18 ± 3.96 mg/dL) and in EX+SD mice (p<0.05) (81.90 ± 3.93 mg/dL) as compared to the control group (137.4 ± 8.62 mg/dL). Serum cholesterol was uniform in all groups. Glycogen was elevated in the liver of EX (4.77 ± 0.35 mg/100mg) mice as well as in muscle (0.6474 ± 0.0067 mg/100mg), although the latter had only a moderate increase. A degree of homogeneity was found among the other groups. Hepatic triglycerides were reduced in SD (8.32 ± 0.95 mg/100mg) and high in EX + SD mice (20.72 ± 2.67 mg/100mg). Furthermore, hepatic cholesterol was elevated in EX + SD (3.84 ± 0.48 mg/100mg) mice,


Sleep deprivation and association with exercise modify serum glucose, lactate, and triglyceride levels, as well as liver and muscle glycogen stores. Muscle levels were maintained under direct control, as expected. Previous exercise increased cholesterol and trygliceride levels in the liver of SD mice. Our findings suggest that exercise provide a biologic advantage in adverse condition such as extreme sleep deprivation.


Sleep deprivation. Exercise. Metabolism. Glycogen. Cholesterol.


Área Clínica


Bruna Rafaele Diógenes Silva, veralice Meireles Sales Bruin, Paulo Iury Gomes Nunes, Robson Salviano Matos, Pedro Felipe Carvalhedo Bruin, Flavia Almeida Santos, Thiago Medeiros da Costa Daniele